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The Influence of IVT on Endothelial Functions in Functional Medicine, Longevity & Biohacking

Mechanism of Endothelial Stimulation

This IVT provokes rhythmic vasodilation and compression and, in a purely physical way, naturally increases blood flow [1–3, 9]. This improves the macro- and microvascular perfusion parameters of the lower extremity and, as shown experimentally and clinically, increases nutritive oxygen supply [1, 4, 6, 7]. During rhythmic dilation/compression, shear forces arise at the vessel wall; the endothelium converts these mechanical stimuli into increased NO release (vasodilation, anti-atherogenic signaling pathways, activation of endothelial nitric oxide synthase) [1, 3, 8, 9].

Nitric Oxide Release and Vasodilation

A central mechanism of IVT is the stimulation of NO release via arterial shear forces acting on endothelial cells. The endothelium acts as a mechanotransducer that converts shear forces exerted by blood flow into chemical stimuli such as the formation of NO [4–6, 9]. NO is a potent vasodilator that exerts anti-atherogenic as well as anti-proliferative effects on the arterial wall. [8–10]

Microcirculatory Improvements

IVT leads to a significant improvement in microcirculation through several synergistic mechanisms [7–9]. Alternating application of negative pressure induces vasodilation, particularly of venous capillaries, thereby stimulating arterial perfusion. Experimental studies show that vacuum therapy increases capillary diameters and blood volume [10].

Treatment with IVT is associated with marked capillary end-dilatation and capillarization, together with an increase in micro- and macroperfusion in the lower extremities. The widening of the smallest vessels during the negative-pressure phases allows more blood to reach the muscle and results in an increase in arterial blood flow [4].

Protective Mechanisms in Circulatory Disorders

Endothelial Protective Functions: IVT activates multiple endothelial protective functions through the physical stimulation of the vessel wall [9, 12]. Laminar shear forces lead to increased expression of endothelial NO synthase in endothelial cells. These shear-stress-induced changes are potentially atheroprotective and contribute to vascular health [8, 11].

Vascular Training and Adaptation: Intermittent/negative-pressure applications support edema reduction and improve cutaneous microcirculation [6, 7, 11].

Neurological Protective Responses: The physical change in pressure activates baroreceptors and sympathetic responses, leading to adaptation of the cardiovascular system [13]. When negative pressure is reached rapidly, arterial baroreceptors are activated, which respond to mean arterial pressure [13, 14].

Influence on Cellular Metabolism through Improved Oxygen Supply

Optimization of Nutrient and Oxygen Supply: IVT activates cellular metabolic processes through improved oxygen and nutrient delivery. The increased microcirculation leads to better oxygenation of cells and simultaneously supports the removal of metabolic waste products and toxins. These processes are fundamental for maintaining cellular homeostasis and regeneration [8, 11].

Mitochondrial Function and Energy Metabolism: NO influences myocardial oxygen consumption and can modulate the mitochondrial respiratory chain. Increased microcirculation optimizes mitochondrial respiration and reduces oxidative stress. The improved oxygen supply through IVT can indirectly support mitochondrial function. NO influences myocardial oxygen consumption and can modulate the mitochondrial respiratory chain. Increased microcirculation optimizes mitochondrial respiration and reduces oxidative stress. [8]

Cellular Regeneration and Metabolic Activation: Improved blood flow enhances the removal of metabolic end products and brings in fresh, oxygen- and nutrient-rich blood. This leads to activation of cellular metabolism and supports regenerative processes at the cellular level [11].

Effects on Inflammatory Processes and Tissue Healing

Anti-inflammatory Effects: IVT exhibits anti-inflammatory effects through the modulation of inflammatory processes. In patients with inflammatory diseases, a reduction of the acute inflammatory response by 37.5% was observed. The therapy leads to lower concentrations of pro-inflammatory cytokines in the blood serum [15].

Central cytokines in the regulation of inflammation are IL-1 and TNF, which are considered key pro-inflammatory cytokines. IVT can reduce the release of these inflammatory mediators and contribute to an improved inflammatory status [16].

Tissue Healing and Angiogenesis: IVT accelerates wound healing through several synergistic mechanisms. Improved microcirculation increases oxygen supply to the wound area, which is essential for healing processes [17]. The therapy stimulates endothelial proliferation and angiogenesis, restores the integrity of the capillary basement membrane, and reduces vascular permeability [18, 19].

Studies show that vacuum therapy promotes the formation of granulation tissue and mechanically approximates wound edges [19, 20].

Lymphatic Drainage and Edema Reduction: IVT acts as an effective lymph drainage method through its physiological action on the “removal of lymph-obligatory loads.” This leads to pronounced reduction of edema and supports tissue regeneration [11].

Delay of Age-Related Cellular Damage through Improved Circulation

Reduction of Oxidative Stress: Oxidative stress arises from an excess of free radicals, which are responsible for cellular damage and are associated with skin aging, inflammation, and disease. IVT can reduce age-related cellular damage over the long term by improving microcirculation and oxygen supply [8].

Antioxidants play an important role in neutralizing free radicals, and optimized blood flow can improve the supply of these protective substances [8].

Vascular Aging and Endothelial Function: Decreased activity or dysfunction of endothelial NO synthase promotes the development of vascular diseases such as atherosclerosis. By stimulating NO release, IVT can counteract these aging processes [8].

Cellular Regeneration and Repair Mechanisms: The physical stimulation by IVT activates repair mechanisms and supports the maintenance of cellular integrity. The therapy can slow the accumulation of cellular damage and promote cellular regeneration. Hypoxic conditions, as they occur in vacuum therapy, can promote stem cell mobilization and support regenerative processes [4].

Systemic Anti-Aging Effects: IVT shows systemic effects on health by optimizing fundamental transport pathways for oxygen, nutrients, and waste products. Its non-invasive nature and the absence of side effects make it an attractive option in preventive and regenerative medicine. The technology ideally complements other longevity interventions, as it operates at the physical level and establishes the basic prerequisites for cellular health.

References

  1. Sundby ØH, Hennig T, Hanssen T-A, Hisdal J. Application of intermittent negative pressure on the lower leg and foot increases foot perfusion in healthy volunteers. Physiol Rep. 2016;4(17):e12911. doi:10.14814/phy2.12911.
  2. Sundby ØH, Hennig T, Hisdal J. Acute effects of lower limb intermittent negative pressure on macro- and microcirculation in peripheral arterial disease. PLoS One. 2017;12(6):e0179001. doi:10.1371/journal.pone.0179001.
  3. Hoel H, Rossebø AB, Halle M, et al. Intermittent negative pressure therapy improves walking capacity in intermittent claudication: randomized controlled trial. J Vasc Surg. 2021;73(5):1690–1700. doi:10.1016/j.jvs.2020.10.024.
  4. Hageman D, et al. Vacuum therapy in patients with intermittent claudication: randomized controlled trial. J Vasc Surg. 2020;72(6):2105–2113. doi:10.1016/j.jvs.2019.08.239.
  5. Malmsjö M, Ingemansson R, Martin R, Huddleston E. The effects of variable, intermittent and continuous negative pressure on wound edge microvascular blood flow. Ann Plast Surg. 2012;68(6 Suppl):S41–S47.
  6. Sogorski A, Lehnhardt M, Görtz O, et al. Intermittent negative pressure therapy and cutaneous microcirculation: review. Front Surg. 2022;9:822122. doi:10.3389/fsurg.2022.822122.
  7. Panayi AC, Leavitt T, Orgill DP. Evidence-based review of negative pressure wound therapy. World J Dermatol. 2017;6(1):1–16. doi:10.5314/wjd.v6.i1.1.
  8. Davies PF. Flow-mediated endothelial mechanotransduction. Physiol Rev. 1995;75(3):519–560. doi:10.1152/physrev.1995.75.3.519.
  9. Thijssen DH, Atkinson CL, Ono K, et al. Sympathetic nervous system activation, arterial shear rate, and flow-mediated dilation. J Appl Physiol (1985). 2014;116(10):1300–1307. doi:10.1152/japplphysiol.00110.2014.
  10. Trinity JD, Groot HJ, Layec G, et al. Passive leg movement and nitric oxide-mediated vascular function: the impact of age. Am J Physiol Heart Circ Physiol. 2015;308(6):H672–H679. doi:10.1152/ajpheart.00806.2014.
  11. Campisi CC, Ryan M, Campisi CS, Boccardo F. Intermittent Negative Pressure Therapy in the combined treatment of peripheral lymphedema. Lymphology. 2015;48(4):197–204.
  12. European Society for Vascular Surgery (ESVS). 2024 Clinical Practice Guidelines on the management of asymptomatic peripheral arterial disease and intermittent claudication. Eur J Vasc Endovasc Surg. 2024;67(1):1–116. doi:10.1016/j.ejvs.2023.08.067.
  13. Leach OK, Gifford JR, Mack GW. Rapid onset vasodilation during baroreceptor loading and unloading. Am J Physiol Regul Integr Comp Physiol. 2023;325(5):R568–R575.
  14. Mikhailov VM, Gurfinkel’ YI, Kudutkina MI, Ushakov BB. Investigation into microcirculation at rest and during the LBNP test. Aviakosm Ekolog Med. 2005;39(5):53–58.
  15. Zapenko A. Retrospective data analysis of 10 years of clinical application of “negative pressure wound therapy” in Germany [dissertation]. Essen (DE): University of Duisburg-Essen, Medical Faculty; [date unknown].
  16. Kettner-Buhrow D. Investigations on RNAi-mediated suppression of effector molecules in inflammation [dissertation]. Hannover (DE): Gottfried Wilhelm Leibniz University Hannover, Faculty of Natural Sciences; 2006.
  17. Worms J, Hoang LPA, Quester W, Stratmann B, Tschöpe D. Effekt der intermittierenden Unterdrucktherapie bei einer älteren Patientin mit diabetischem Fußsyndrom und kritischer Ischämie. Diabetol Stoffwechs. 2015;10(Suppl 1):P283. doi:10.1055/s-0035-1549789.
  18. European Society of Cardiology (ESC). Consensus on exercise therapy for chronic symptomatic peripheral arterial disease. Eur J Prev Cardiol. 2024;31(12):1588–1604. doi:10.1093/eurheartj/ehad734.
  19. Lindsay B. Vacumed Therapy for Peripheral Arterial Disease and Leg Disease. Clinical Evaluation Report – VACUMED® Intermittent Vacuum Therapy. Internal document. Weyergans High Care AG, Düren; 2025.
  20. Crkvenac Gregorek A, Pavić P, Meštrović T, et al. Vacumed IVT in chronic wound healing. Presented at: Online Conference on Vascular Surgery; 29 Aug 2022; Zagreb, Croatia. Weyergans High Care AG; 2025.

Intermittent Vacuum Therapy (IVT) with Integrated Photobiostimulation Module (Red/NIR)

Version: Master (EN) · As of: 06.10.2025

Summary

The Intermittent Vacuum Therapy (IVT) of the Vacustyler® Avantgarde operates with rhythmically alternating negative and positive pressure phases on the lower body. The resulting shear forces activate the eNOS/NO axis, improve microcirculation, promote venous return and lymphatic flow, and modulate inflammatory processes. The integrated photobiostimulation module (Red/NIR ≈ 630–850 nm) complements IVT at the cellular level via mitochondrial mechanisms. In longevity medicine, IVT primarily addresses the Hallmarks “Altered intercellular communication,” “Chronic inflammation,” “Mitochondrial dysfunction,” and indirectly “Cellular senescence” [1–6,10–16].

Key Points (at a Glance)

Background & Objective

IVT is a non-invasive procedure with roots in space/sports and rehabilitation medicine. The objective is to improve perfusion, endothelial function, and fluid dynamics of the lower extremities – with relevance for regeneration, wound healing, venous-lymphatic disorders, as well as metabolic and aesthetic applications [1–9,14–16].

Mechanism of Action

1) Pressure Phases (IVT): Alternating negative and positive pressure phases (typ. 2–30 s) generate shear forces at the vascular endothelium, increase transmural pressure gradients, and shift blood/lymph volume. Negative pressure leads to vasodilation/volume increase, normal/positive pressure to return flow promotion [6–9,10–12].

2) Endothelium & NO: Mechanotransduction increases eNOS activity and NO release → improved vascular elasticity, anti-atherogenic effects, better FMD [10–12].

3) Microcirculation: Capillary recruitment, flow velocity, and local blood volume increase; tissue perfusion improves measurably [6–9,13].

4) Lymphatic Activation: The cyclic volume shift relieves the lymphatic system; edema and tissue pressure decrease [14–16].

5) Neuromodulation: Baroreceptor and sympathovagal responses support orthostatic regulation [20–21].

6) Integrated Photobiostimulation (Red/NIR): The LED module (≈ 630–850 nm) is integrated into the device and can be activated in parallel. Target mechanisms: cytochrome c oxidase activation, ATP↑, ROS/inflammation↓, fibroblast activation, and tissue tightening [17–19].

Evidence Overview (Clinical Effects)

Indications & Contraindications

Common Indications: Chronic microcirculatory disorders; venous insufficiency, lymphedema/edema; wound healing, postoperative and athletic regeneration; skin/tissue tightening (with activated PBM module); “Longevity baseline”: perfusion- and metabolism-supporting base intervention [6–9,14–19].

Contraindications (Examples): Acute thrombosis, decompensated heart failure; acute infections/fever, active bleeding/wounds (without protection); pregnancy (center-dependent); always seek medical clearance.

Protocols & Parameters

Standard Session: Duration: 20–30 min (often 25 min). Cycles: negative/positive pressure 2–30 s each. Frequency: 2–4×/week; series of 6–12; maintenance 1×/week. PBM: activate in parallel according to goal (Red/NIR).

GoalIVT FocusPBM UseNotes
Microcirculation/RegenerationLonger negative-pressure phasesRed+NIRAdapt to training/surgical window
Lymph/EdemaBalanced cyclesOptionalCombine with compression & elevation
Endothelial FunctionModerate cyclesOptionalTrack FMD/functional measures
Skin/Tissue (Tightening)Standardized cyclesRed+NIR activeSkin status/photo documentation

Parameters should be individualized according to device and patient specifications.

Safety & Monitoring

Non-invasive, generally well tolerated. Monitoring: blood pressure, comfort, orthostatic symptoms. Dose finding: stepwise, especially in cardiovascular patients [10,20–21].

Synergies

Integrated PBM (Red/NIR): simultaneous application enhances effects on mitochondria, inflammation, and intercellular communication [17–19].

Cryotherapy/Hormesis: IVT (perfusion basis) + cold trigger combined before/after IVT depending on goal.

Hallmarks Mapping

Technical Notes & Operation

PBM module: can be activated with any program; button red = active, gray = inactive; auto-stop with program end. Hygiene: when combining IVT+PBM, use foil trousers (single use). Pressure ranges: negative pressure typ. −20 to −70 mbar (device-dependent). Details see device manual.

Cost-Effectiveness (Brief)

Series and maintenance protocols enable continuous care at low operating costs; cross-selling with PBM, training, nutrition/lifestyle programs is advisable.

References

  1. López-Otín C, Kroemer G. Hallmarks of aging: An expanding universe. Cell. 2023;186(2):243–78. doi:10.1016/j.cell.2022.12.021.
  2. López-Otín C, Blasco MA, Partridge L, Serrano M, Kroemer G. The hallmarks of aging. Cell. 2013;153(6):1194–213. doi:10.1016/j.cell.2013.05.039.
  3. Mučka S, Mieszkowski J, Niewiadomski W, et al. Endothelial function assessment by flow-mediated dilation. Int J Environ Res Public Health. 2022;19(17):10672. doi:10.3390/ijerph191710672.
  4. Ferrucci L, Fabbri E. Inflammaging: chronic inflammation in aging. Nat Rev Cardiol. 2018;15(9):505–22. doi:10.1038/s41569-018-0064-2.
  5. Sundby ØH, Høiseth LØ, Rognmo Ø, Hisdal J. Intermittent negative pressure on lower limbs in limb ischemia & hard-to-heal ulcers: case reports. Physiol Rep. 2016;4(21):e13012. doi:10.14814/phy2.13012.
  6. Green DJ, Hopman MTE, Padilla J, Laughlin MH, Thijssen DHJ. Vascular adaptation to exercise training. Physiol Rev. 2017;97(2):495–528. doi:10.1152/physrev.00014.2016.
  7. Thijssen DHJ, Black MA, Pyke KE, et al. Recommendations for assessment of flow-mediated dilation. Eur Heart J. 2019;40(30):2534–47. doi:10.1093/eurheartj/ehz350.
  8. Moncada S, Higgs EA. Nitric oxide and the vascular endothelium. Pharmacol Rev. 2006;58(3):315–41. doi:10.1124/pr.58.3.6.
  9. Green DJ, Jones H, Thijssen D, Cable NT, Atkinson G. FMD and cardiovascular event prediction. Hypertension. 2011;57(3):363–9. doi:10.1161/HYPERTENSIONAHA.110.167015.
  10. Hamblin MR. Anti-inflammatory effects of photobiomodulation. AIMS Biophys. 2017;4(3):337–61. doi:10.3934/biophy.2017.3.337.
  11. de Freitas LF, Hamblin MR. Proposed mechanisms of photobiomodulation. IEEE J Sel Top Quantum Electron. 2016;22(3):7000417. doi:10.1109/JSTQE.2016.2561201.
  12. Chung H, Dai T, Sharma SK, et al. Low-level light therapy: mechanisms and clinical applications. Ann Biomed Eng. 2012;40(2):516–33. doi:10.1007/s10439-011-0454-7.
  13. Sogorski A, Nonn L, Berger F, et al. Negative-pressure device cyclic application enhances cutaneous microcirculation. Int J Mol Sci. 2022;23(4):2195. doi:10.3390/ijms23042195.
  14. Campisi CC, Ryan M, Boccardo F, et al. Intermittent negative pressure therapy for peripheral lymphedema. Lymphology. 2015;48(2):80–7.
  15. Dunn N, Williams A, Vowden P, Vowden K. Intermittent pneumatic compression for lymphedema: systematic review. J Pers Med. 2022;12(10):1681. doi:10.3390/jpm12101681.
  16. Kim Y, Yoo J, Jang H, et al. Home-based intermittent pneumatic compression in chronic leg lymphedema. Healthcare (Basel). 2022;10(4):638. doi:10.3390/healthcare10040638.
  17. ClinicalTrials.gov. Intermittent Negative Pressure in PAD (NCT03640676).
  18. ClinicalTrials.gov. Effect of Intermittent Pressure in PAD (NCT03854097).
  19. Täger CD, Hager S, Steinsträßer L, et al. Negative pressure wound therapy – microvascular perfusion & microenvironment. Int J Mol Sci. 2024;25(9):4739. doi:10.3390/ijms25094739.
  20. Parati G, Saul JP, Di Rienzo M, Mancia G. Spectral analysis of BP/HR variability. Hypertension. 1995;25(6):1276–86. doi:10.1161/01.HYP.25.6.1276.
  21. Weyergans R. Handbuch Vacustyler® Avantgarde V03. 2025. Device manual (manufacturer documentation).